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|Title: ||Cytotoxic and wild type P53-Promoting activities of selected Zingiberaceae species in colon cancer cell lines|
|Authors: ||Fathiah Abdullah|
|Issue Date: ||2009 |
|Publisher: ||University Malaya|
|Abstract: ||The Zingiberaceae family is distributed worldwide and is acknowledged not only as an essential food but has been used throughout history for their medicinal effects. In the present study, 30 crude petroleum ether, chloroform and methanol extracts of ten selected Zingiberaceae species namely Alpinia galanga, Boesenbergia rotunda, Curcuma aeruginosa, Curcuma domestica, Curcuma mangga, Curcuma xanthorriza, Kaempferia galanga, Zingiber montanum, Zingiber officinale and Zingiber zerumbet were studied. The crude extracts of these plants were screened for their cytotoxic activity (with and without 5-fu) against human colon cancer derived- HCT-116 and HT29 cells and for their wild type p53 (wt-p53) promoting activity in HCT-116 and HT29 cell lines.
The crude extracts of selected Zingiberaceae were screened at varying concentrations ranging from 1 μg/ml to 100 μg/ml for their cytotoxic activity using the neutral red (NR) cytotoxicity assay. Results indicated that the percentage of inhibition in HCT-116 and HT29 cells increased with the increasing concentration of extracts.
The best extracts from selected Zingiberaceae extracts that showed active cytotoxic activity were further tested with 5-Fluorouracil (5-Fu) against HCT-116 and HT29 cells using neutral red (NR) assay. The combination of 5-Fu with all the selected extracts exhibited better cytotoxic effect and gave the better values of IC50 as compared to 5-Fu alone. Synergy with Zingiberaceae extracts was also seen at low 5-Fu concentrations.
In the current study, crude petroleum ether, chloroform and methanol extracts of ten selected Zingiberaceae species were analyzed qualitatively for wt-p53 proliferation in colon cancer cell lines, HCT-116 and HT29. The cells were then treated independently with each of the crude extracts at varying concentrations of 0, 1, 10, 25, 50 and 100 μg/ml for 72 hours at 37oC in the 5 % carbon dioxide (CO2) incubator. The 3-step indirect avidin-biotin immunoperoxidase technique was subsequently used to examine the expression of wt-p53 in both cell lines. Results showed that the expression of wt-p53 increased in HCT-116 and HT29 cells treated with crude extracts of the selected Zingiberaceae species.
The results of the present study indicated that Zingiber zerumbet, Alpinia galanga and Zingiber officinale extracts showed significant cytotoxic effect and wt-p53 promoting activity. Further studies should be carried out to isolate and identify the active compound(s) and to elucidate their mechanism of action.|
|Description: ||Dissertation -- Faculty of Science, University of Malaya, 2009.|
|Appears in Collections:||Masters Dissertations : Science|